Peyton Elizabeth Lee

Born in New York City, she moved to Manhattan Beach, California, and began acting at the age of 10.

Lee is half Chinese on her father's side; her father is actor Andrew Tinpo Lee, whom she describes as "one of the major reasons why I have become an actor." Her mother, Jennifer Dormer Lee, is a psychologist. She has an older sister and a younger brother.

In September 2022, Lee enrolled at Columbia University in New York City.Prior to Andi Mack, Lee featured in numerous commercials for Sprint, Carnival Cruises, Petco, Apple's iPhone 6s, and JCPenney. She also appeared in three episodes of Shameless as a "girl soldier" and one episode of Scandal as "Violet", a spelling bee champion. In December 2014, Lee performed in Debbie Allen's Hot Chocolate Nutcracker which was performed at the Royce Hall at UCLA.

Andi Mack premiered on Disney Channel on April 7, 2017. When asked about casting Lee in the lead role, Andi Mack executive producer Terri Minsky said she "liked that Lee did not look as if she had fallen off a child-star assembly line", later stating that "What's really great about Peyton is that she can easily handle both the drama and the comedy of this character." On January 9, 2018, it was announced that Lee would be joining the Disney Junior animated series The Lion Guard as Rani, appearing in nine episodes from the third season.

On January 14, 2021, it was announced that Lee would play the title role in Doogie Kameāloha, M.D., a reboot of Doogie Howser, M.D. that airs on Disney+ which ran from September 8, 2021 to March 31, 2023.

In August 2022, Lee was cast in the animated feature film Wildwood which is a film adaptation of the 2011 novel by Colin Meloy with the film set to be released in 2025.

Lee starred in the lead role of Mandy Yang in the television film Prom Pact alongside Milo Manheim which was released on March 30, 2023.

In June 2024, Lee was cast in the Hulu original film entitled, "Carved" which was released on October 21, 2024.