Melissa Villaseñor

Villaseñor was born in Whittier, California, in Los Angeles County, on October 9, 1987, the daughter of Mexican parents Guadalupe "Lupe" Peralta and Miguel "Michael" Humberto Villaseñor. She is of Spanish, Basque, and indigenous Mexican descent, with roots in Jalisco and Aguascalientes, Mexico. She also has 2% Sub-Saharan African DNA. Villaseñor and actor Mario Lopez—whose Mexican ancestry was also explored on the same episode of Finding Your Roots—are both distantly related to director Alejandro González Iñárritu.

She attended the Catholic Ramona Convent Secondary School in Alhambra, California. She briefly attended Fullerton College. At 15, she started performing stand-up comedy at the Laugh Factory Comedy Camp in Hollywood. Villaseñor was a semifinalist on the sixth season of America's Got Talent. Previously, she was selected for the New Faces lineup in the 2010 Just for Laughs Montreal Comedy Festival. She has also done voice work for the animated shows Adventure Time, Family Guy, and TripTank. She appeared on the second season of HBO's Crashing.

Villaseñor first auditioned for the late night sketch comedy/variety show Saturday Night Live in 2009, where she did several impressions but was not hired. Villaseñor was introduced as a featured player on the October 1, 2016 episode of Saturday Night Live, alongside Mikey Day and Alex Moffat. Her debut was an impression of Sarah Silverman in the Family Feud: Political Edition sketch. She is the second Latina cast member after Noël Wells, who is a quarter Mexican, and the first Latina to be promoted to repertory status. Villaseñor appeared between the show's 42nd and 47th seasons.

After she became a cast member, she deleted almost 2,000 old tweets, that she had posted mostly in 2010 and 2011, which some critics called racist. SNL and Villaseñor initially declined to comment on the accusation, but in September 2018, Villaseñor explained that she was "trying to be edgy" when she posted them, and that she did not regret deleting them. She told NPR's Weekend Edition Saturday, "I'm flawed like everyone else, and yeah, I just wanted to make sure I was perfect."

She was promoted to the cast's repertory players in season 44 in 2018. Villaseñor left the show at the end of the 47th season in 2022, after six years as a cast member.

Her debut album, Dreamer, a pop-punk/power pop-influenced work, was released in October 2019. She is also an illustrator.

She helped Rob Cantor create his viral video "29 Celebrity Impressions, 1 Original Song" to promote his album Not a Trampoline, in which she sings impressions of Britney Spears, Christina Aguilera, and Björk.

She hosted the 2021 Independent Spirit Awards, and voiced Robin in the radio drama podcast Batman: The Audio Adventures.

In 2023, it was announced that she would voice Nellie Ramírez-Humphrey in Primos.

Villaseñor describes herself as a comedian and impressionist. She has performed impressions for America's Got Talent, First Impressions, her YouTube channel, and SNL including Björk, Dolly Parton, Owen Wilson, Miley Cyrus, Gwen Stefani, Jennifer Lopez, Kate McKinnon, Kristen Wiig, Christina Aguilera, Britney Spears, Julia Louis-Dreyfus, Ana Navarro, Sia, Natalie Portman, Macaulay Culkin, Sarah Silverman, Lady Gaga, Billie Eilish, Dua Lipa and Alexandria Ocasio-Cortez.